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Dna gel electrophorosis
Title: Dna gel electrophorosis
Category: Science & Technology
Details: Words: 569 | Pages: 2.4 (approximately 235 words/page)
Dna gel electrophorosis
Introduction:
DNA, Deoxyribonucleic acid, is a double stranded, helical nucleic
acid molecule which determines inherited structure of a protein. The
“steps” are made of bases: adenine, guanine, cytosine, and thymine. The
sides are sugar and phosphate molecules. Restriction enzymes are
enzymes that cut DNA at restriction sites, leaving fragments blunt or
sticky. The restriction fragments are separated using a technique called
gel electrophoresis.
DNA has a negative charge so when an electrical charge is
applied …showed first 75 words of 569 total…
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…showed last 75 words of 569 total…turned off after the bands migrated near the end of
the gel and the top of the electrophoresis chamber was removed. We
removed the gel from the gel casting tray and examined it under a light
box and compared it to the ideal gel (figure1-2).
Bibliography
References:
Restriction Enzymes: Cleavage of DNA lab
University of Illinois. (1999). Experiment 2
Gel Electrophoresis of DNA. In
Molecular Biology Cyberlab, online:
Http://www.life.uluc.edu/molbio/geldigest/electro.html
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